The Neuroanatomy SecondLook™ application is a study aid that provides a series of neuroanatomical images for users to self-test their level of knowledge and ability to recognize structures within the human central nervous system. The images in the Neuroanatomy SecondLookTM application were captured using the University of Michigan plastinated collection. This application was developed with the learner in mind, especially medical, dental, nursing, and other health sciences students, and lab professionals. The SecondLookTM Lite Neuroanatomy sample application is free to download and contains a representative slide from each of the available seven sets. The complete slide sets, covering Gross Neuroanatomy, Cranial Nerves, Diencephalon, Forebrain, Brain Stem, Limbic System, and Neurohistology, may be purchased as a complete mobile application. By taking a "Second Look" at these images, users are able to quickly self-evaluate and test their knowledge of the human central nervous system in order to identify gaps and to be better prepared for tests. This mobile application will also serve as an excellent review tool to prepare for licensing examinations.