The Cytometry Toolkit

1K+
Downloads
Content rating
Everyone
Screenshot image
Screenshot image
Screenshot image
Screenshot image

About this app

The Cytometry Toolkit comprises a number of different calculators that aid in setting up and running cytometers, including sorting flow cytometers.


Features:
*Resolution calculator:
Calculates the S or Separation parameter of two dim populations. Useful if you want a quickly test a new optical filter or a new dye in your assay.

*Q B and S calculator:
This calculator is like the Resolution Calculator by gives much more information such as the Quantum efficiency of the system, the Background and a calculated separation parameter. It also includes the Separation Parameter as reported by the Resolution calculator.

*Simple Lens Calculator:
Is used only for thin spherical lenses. This calculator calculates; the Laser Spot Size at the focus, the Wavelength, the Focal Length or the laser beam’s Diameter at the back of the focusing lens.

*Two Recovery Calculators:
Rmax# and Rmax %. These calculators calculate the recovery from a sort. One simply needs to sample the waste stream to assess the efficiency of the sort. Really useful in assessing if the Break-off Point is optimal and is a non-invasive way of calculating the recovery from rare population sorts without having to sample and count the rare particles.

*Poisson sort Calculator
An extension of the Recovery calculators though usable on it’s own, it will calculate the expected recoveries you should get back after a sort if your sort sample behaves in a Poisson way, that is, your particles in the sample are mono-dispersed. The calculator also reports the sort time.


Feedback/suggestions are always welcome. I will update this periodically with bug fixes and new features for at least two years. I hope you find this useful…
Updated on
Dec 30, 2012

Data safety

Developers can show information here about how their app collects and uses your data. Learn more about data safety
No information available

What's new

• Entry for “Non-Target” changed to “Total” in the Rmax# calculation
• The lens calculation now calculates the Depth of Focus (Rayleigh range)
• Changes reflected in the help menus
• Added Rmax# error handler when inputs are incorrect
• General Corrections in the help menus

Version 1.2.1
• The lens calculator sometimes gave incorrect Depth of Focus now Fixed.
• Fix for Rmax# calculator nows shows the "To Poisson" button after calculation.