New to this Edition:Updated and increased coverage of real time PCR and the mathematics used to measure gene expressionMore sample problems in every chapter for readers to practice concepts
Calculations in Molecular Biology and Biotechnology, Third Edition,helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables.
In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work.Features comprehensive calculations in biotechnology and molecular biology experiments from start to finishProvides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technologyIncludes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the textFeatures new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteinsIncludes more sample problems in every chapter for readers to practice concepts
The book utilizes an ongoing fictional narrative throughout, entertaining students as it provides hands-on learning in order to "crack the case." As two investigators try to solve a missing persons case, each succeeding chapter reveals new characters, new information, and new physical evidence to be processed. A full range of topics are covered, including processing the crime scene, lifting prints, trace and blood evidence, DNA and mtDNA sequencing, ballistics, skeletal remains, and court testimony. Following the storyline, students are introduced to the appropriate science necessary to process the physical evidence, including math, physics, chemistry, and biology.
The final element of each chapter includes a series of cost-effective, field-tested lab activities that train students in processing, analyzing, and documenting the physical evidence revealed in the narrative. Practical and realistic in its approach, this book enables students to understand how forensic science operates in the real world.
*A comprehensive guide to calculations for a wide variety of problems encountered in the basic research laboratory.
* Example calculations are worked through from start to finish in easy-to-follow steps
* Key chapters devoted to calculations encountered when working with bacteria, phage, PCR, radioisotopes, recombinant DNA, centrifugation, oligonucleotides, protein, and forensic science.
*Written for students and laboratory technicians but a useful reference for the more experienced researcher.
*A valuable teaching resource.