The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
All Martes have been documented to use forested habitats and 6 species (excluding the stone marten) are generally considered to require complex mid- to late-successional forests throughout much of their geographic ranges. All species in the genus require complex horizontal and vertical structure to provide escape cover protection from predators, habitat for their prey, access to food resources, and protection from the elements. Martens and the fisher have high metabolic rates, have large spatial requirements, have high surface area to volume ratios for animals that often inhabit high latitudes, and often require among the largest home range areas per unit body weight of any group of mammals. Resulting from these unique life history characteristics, this genus is particularly sensitive to human influences on their habitats, including habitat loss, stand-scale simplification of forest structure via some forms of logging, and landscape-scale effects of habitat fragmentation. Given their strong associations with structural complexity in forests, martens and the fisher are often considered as useful barometers of forest health and have been used as ecological indicators, flagship, and umbrella species in different parts of the world. Thus, efforts to successfully conserve and manage martens and fishers are associated with the ecological fates of other forest dependent species and can greatly influence ecosystem integrity within forests that are increasingly shared among wildlife and humans.
We have made great strides in our fundamental understanding of how animals with these unique life history traits perceive and utilize habitats, respond to habitat change, and how their populations function and perform under different forms of human management and mismanagement. This knowledge enhances our basic understanding of all species of Martes and will help us to achieve the goal of conserving viable populations and representative distributions of the world's Martes, their habitats, and associated ecological communities in our new millennium.
This book provides an overview of mouse development, spanning from oocytes before fertilization to the state-of-the-art description of embryonic and adult stem cells. The chapters, written by the leading specialists in the field, deal with the most recent discoveries in this extremely fast-developing area of research.
This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.