the drama of the front lines.”
-Richard Danzig, former secretary of the navy
The first major bioterror event in the United States-the anthrax attacks in October 2001-was a clarion call for scientists who work with “hot” agents to find ways of protecting civilian populations against biological weapons. In The Demon in the Freezer, his first nonfiction book since The Hot Zone, a #1 New York Times bestseller, Richard Preston takes us into the heart of Usamriid, the United States Army Medical Research Institute of Infectious Diseases at Fort Detrick, Maryland, once the headquarters of the U.S. biological weapons program and now the epicenter of national biodefense.
Peter Jahrling, the top scientist at Usamriid, a wry virologist who cut his teeth on Ebola, one of the world’s most lethal emerging viruses, has ORCON security clearance that gives him access to top secret information on bioweapons. His most urgent priority is to develop a drug that will take on smallpox-and win. Eradicated from the planet in 1979 in one of the great triumphs of modern science, the smallpox virus now resides, officially, in only two high-security freezers-at the Centers for Disease Control in Atlanta and in Siberia, at a Russian virology institute called Vector. But the demon in the freezer has been set loose. It is almost certain that illegal stocks are in the possession of hostile states, including Iraq and North Korea. Jahrling is haunted by the thought that biologists in secret labs are using genetic engineering to create a new superpox virus, a smallpox resistant to all vaccines.
Usamriid went into a state of Delta Alert on September 11 and activated its emergency response teams when the first anthrax letters were opened in New York and Washington, D.C. Preston reports, in unprecedented detail, on the government’s response to the attacks and takes us into the ongoing FBI investigation. His story is based on interviews with top-level FBI agents and with Dr. Steven Hatfill.
Jahrling is leading a team of scientists doing controversial experiments with live smallpox virus at CDC. Preston takes us into the lab where Jahrling is reawakening smallpox and explains, with cool and devastating precision, what may be at stake if his last bold experiment fails.
This expansive text pulls together the numerous fields of study encompassed by biogeochemistry to analyze the increasing demands of the growing human population on limited resources and the resulting changes in the planet's chemical makeup.
The book helps students extrapolate small-scale examples to the global level, and also discusses the instrumentation being used by NASA and its role in studies of global change. With extensive cross-referencing of chapters, figures and tables, and an interdisciplinary coverage of the topic at hand, this updated edition provides an excellent framework for courses examining global change and environmental chemistry, and is also a useful self-study guide.Winner of a 2014 Texty Award from the Text and Academic Authors AssociationCalculates and compares the effects of industrial emissions, land clearing, agriculture, and rising population on Earth's chemistrySynthesizes the global cycles of carbon, nitrogen, phosphorous, and sulfur, and suggests the best current budgets for atmospheric gases such as ammonia, nitrous oxide, dimethyl sulfide, and carbonyl sulfideIncludes an extensive review and up-to-date synthesis of the current literature on the Earth's biogeochemistry
This edition includes greatly expanded focus on stem cells, including adult and embryonic stem cells and progenitor populations that may soon lead to new tissue engineering therapies for heart disease, diabetes, and a wide variety of other diseases that afflict humanity. This up-to-date coverage of stem cell biology and other emerging technologies is complemented by a series of new chapters on recent clinical experience in applying tissue engineering. The result is a comprehensive textbook that we believe will be useful to students and experts alike.
New to this edition:
*Includes new chapters on biomaterial-protein interactions, nanocomposite and three-dimensional scaffolds, skin substitutes, spinal cord, vision enhancement, and heart valves
*Expanded coverage of adult and embryonic stem cells of the cardiovascular, hematopoietic, musculoskeletal, nervous, and other organ systems
Life is the most extraordinary phenomenon in the known universe; but how did it come to be? Even in an age of cloning and artificial biology, the remarkable truth remains: nobody has ever made anything living entirely out of dead material. Life remains the only way to make life. Are we still missing a vital ingredient in its creation?
Using first-hand experience at the cutting edge of science, Jim Al-Khalili and Johnjoe Macfadden reveal that missing ingredient to be quantum mechanics. Drawing on recent ground-breaking experiments around the world, each chapter in Life on the Edge illustrates one of life's puzzles: How do migrating birds know where to go? How do we really smell the scent of a rose? How do our genes copy themselves with such precision? Life on the Edge accessibly reveals how quantum mechanics can answer these probing questions of the universe.
Guiding the reader through the rapidly unfolding discoveries of the last few years, Al-Khalili and McFadden describe the explosive new field of quantum biology and its potentially revolutionary applications, while offering insights into the biggest puzzle of all: what is life? As they brilliantly demonstrate in these groundbreaking pages, life exists on the quantum edge.
– Winner, Stephen Hawking Medal for Science Communication
This new edition discusses biological systems that can be analyzed quantitatively, and how advances in the life sciences have been aided by the knowledge of physical or engineering analysis techniques. The volume is organized into 18 chapters encompassing thermodynamics, electricity, optics, sound, solid mechanics, fluid mechanics, and atomic and nuclear physics. Each chapter provides a brief review of the background physics before focusing on the applications of physics to biology and medicine. Topics range from the role of diffusion in the functioning of cells to the effect of surface tension on the growth of plants in soil and the conduction of impulses along the nervous system. Each section contains problems that explore and expand some of the concepts. The text includes many figures, examples and illustrative problems and appendices which provide convenient access to the most important concepts of mechanics, electricity, and optics in the body.
Physics in Biology and Medicine will be a valuable resource for students and professors of physics, biology, and medicine, as well as for applied health workers.Provides practical techniques for applying knowledge of physics to the study of living systemsPresents material in a straight forward manner requiring very little background in physics or biologyIncludes many figures, examples and illustrative problems and appendices which provide convenient access to the most important concepts of mechanics, electricity, and optics in the body
The Olympics are the world's most prestigious stage for athletic competition. Fans both casual and hardcore tune in religiously every few years to watch as men and women push themselves to the limits of human performance. But what makes a champion? Is it genetics? Hours of training? A psychological advantage? Of all the athletes who dedicate their lives - and bodies - to achieving that perfect moment of triumph, why will one person or team win out over another? Science has some compelling answers, and in this book, The Science of Sports: Winning in the Olympics, Scientific American explores this topic from various angles. Beginning with Section 1: The Psychology of Winning, the book opens with a look inside the mind of an elite athlete and tackles questions of how to face a rivalry or maintain a positive attitude in the face of defeat. Other sections discuss the sticky issues surrounding genetic advantages and physical prowess, drugs and doping, injury and recovery, and - finally - the latest scientific advice for the rest of us mere mortals to be fit and healthy. You'll find both inspiration and answers in this indispensable book from the editors of Scientific American, the leading authority on science, technology and innovation.
Interested in unraveling the physics of living things? Here's your starting point. Biophysics Demystified is the fast and easy way to understand this fascinating topic.
Written in a step-by-step format, this practical guide begins with an introduction to the science of biophysics, covering biophysical techniques and applications. Next, you'll learn the principles of physics, biology, and chemistry required to understand biophysics, including free energy, entropy, and statistical mechanics. Biomolecules and the forces that influence their structure and conformation are also covered, as are protein, nucleic acid, and membrane biophysics. Detailed examples and concise explanations make it easy to understand the material, and end-of-chapter quizzes and a final exam help reinforce key concepts.
It's a no-brainer! You'll get:Molecular, subcellular, physiological, anatomical, and environmental biophysics The laws of thermodynamics as they apply to biophysical systems Forces affecting conformation in biological molecules The composition and structure of carbohydrates, lipids, proteins, and nucleic acids The fluid mosaic model
Simple enough for a beginner, but challenging enough for an advanced student, Biophysics Demystified makes this interdisciplinary subject easy to master.
One of the most diverse of modern scientific disciplines, biophysics applies methods and technologies from physics to the study of biological systems and phenomena, from the human nervous system to soil erosion to global warming. What are the best options for satisfying the world's growing energy demands? How can we feed the world's growing population? How can we contain, or reverse, global warming? How can we vouchsafe a plentiful supply of potable water for future generations? These are among the critical questions to which biophysicists work to provide answers.Biophysics courses are increasingly taken by students of biology, physics, chemistry, biochemistry, physiology, statistics, bioengineering, neuroscience, computer science, pharmacology, agriculture,and many more Provides a friendly, unintimidating overview of the material covered in a typical college-level biophysics course A one-stop reference, course supplement and exam preparation tool for university students currently enrolled in an introductory biophysics courses An indispensable resource for those studying the natural sciences, biological sciences, and physics, as well as math, statistics,computer science, pharmacology and many other disciplines The current job market for people well versed in biophysics is very strong, and biophysics is currently listed as one of the fast-growing occupations in the North America
The handbook begins with a series of historical vignettes of pioneers from the last two centuries. It also presents the fundamentals of physics and biology as applied to photomedicine. It next examines conditions and diseases caused by light, including skin cancer, dermatoses, and immunosuppression.
The remainder of the book focuses on the most important clinical therapeutic applications of different kinds of light that vary in both wavelength and intensity. The book discusses ultraviolet phototherapy for skin diseases and infections and presents the basic science of photodynamic therapy and its use in cancer therapy and other medical specialties. It then covers mechanistic studies and clinical applications of low-level laser (light) therapy as well as the use of high power or surgical laser therapy in specialties, such as dentistry and dermatology. The book concludes with a collection of miscellaneous types of phototherapy.
This concise introductory paperback surveys and relates basic physics to living systems. It discusses biological systems that can be analyzed quantitatively, and how advances in the life sciences have been aided by the knowledge of physical or engineering analysis techniques.
This text is designed for premed students, doctors, nurses, physiologists, or other applied health workers, and other individuals who wish to understand the nature of the mechanics of our bodies.
- Provides practical techniques for applying knowledge of physics to the study of living systems
- Presents material in a straight forward manner requiring very little background in physics or biology
- Includes many figures, examples and illustrative problems and appendices which provide convenient access to the most important concepts of mechanics, electricity, and optics
Every brain begins as a female brain. It only becomes male eight weeks after conception, when excess testosterone shrinks the communications center, reduces the hearing cortex, and makes the part of the brain that processes sex twice as large.
Louann Brizendine, M.D. is a pioneering neuropsychiatrist who brings together the latest findings to show how the unique structure of the female brain determines how women think, what they value, how they communicate, and whom they’ll love. Brizendine reveals the neurological explanations behind why
• A woman remembers fights that a man insists never happened
• A teen girl is so obsessed with her looks and talking on the phone
• Thoughts about sex enter a woman’s brain once every couple of days but enter a man’s brain about once every minute
• A woman knows what people are feeling, while a man can’t spot an emotion unless somebody cries or threatens bodily harm
• A woman over 50 is more likely to initiate divorce than a man
Women will come away from this book knowing that they have a lean, mean communicating machine. Men will develop a serious case of brain envy.
As advances in algorithms and computer technology have bolstered the digital signal processing capabilities of real-time sonar, radar, and non-invasive medical diagnostics systems, cutting-edge military and defense research has established conceptual similarities in these areas. Now civilian enterprises can use government innovations to facilitate optimal functionality of complex real-time systems. Advanced Signal Processing details a cost-efficient generic processing structure that exploits these commonalities to benefit commercial applications.
Learn from a Renowned Defense Scientist, Researcher, and Innovator
The author preserves the mathematical focus and key information from the first edition that provided invaluable coverage of topics including adaptive systems, advanced beamformers, and volume visualization methods in medicine. Integrating the best features of non-linear and conventional algorithms and explaining their application in PC-based architectures, this text contains new data on:
Advances in biometrics, image segmentation, registration, and fusion techniques for 3D/4D ultrasound, CT, and MRI
Fully digital 3D/ (4D: 3D+time) ultrasound system technology, computing architecture requirements, and relevant implementation issues
State-of-the-art non-invasive medical procedures, non-destructive 3D tomography imaging and biometrics, and monitoring of vital signs
Cardiac motion correction in multi-slice X-ray CT imaging
Space-time adaptive processing and detection of targets interference-intense backgrounds comprised of clutter and jamming
With its detailed explanation of adaptive, synthetic-aperture, and fusion-processing schemes with near-instantaneous convergence in 2-D and 3-D sensors (including planar, circular, cylindrical, and spherical arrays), the quality and illustration of this text’s concepts and techniques will make it a favored reference.
We will soon be able to meet and exceed the basic needs of every man, woman and child on the planet. Abundance for all is within our grasp. This bold, contrarian view, backed up by exhaustive research, introduces our near-term future, where exponentially growing technologies and three other powerful forces are conspiring to better the lives of billions. An antidote to pessimism by tech entrepreneur turned philanthropist, Peter H. Diamandis and award-winning science writer Steven Kotler.
Since the dawn of humanity, a privileged few have lived in stark contrast to the hardscrabble majority. Conventional wisdom says this gap cannot be closed. But it is closing—fast. The authors document how four forces—exponential technologies, the DIY innovator, the Technophilanthropist, and the Rising Billion—are conspiring to solve our biggest problems. Abundance establishes hard targets for change and lays out a strategic roadmap for governments, industry and entrepreneurs, giving us plenty of reason for optimism.
Examining human need by category—water, food, energy, healthcare, education, freedom—Diamandis and Kotler introduce dozens of innovators making great strides in each area: Larry Page, Steven Hawking, Dean Kamen, Daniel Kahneman, Elon Musk, Bill Joy, Stewart Brand, Jeff Skoll, Ray Kurzweil, Ratan Tata, Craig Venter, among many, many others.
Developed at MIT in collaboration with award-winning high school teachers, BioBuilder teaches the foundational ideas of the emerging synthetic biology field, as well as key aspects of biological engineering that researchers are exploring in labs throughout the world. These lessons will empower teachers and students to explore and be part of solving persistent real-world challenges.Learn the fundamentals of biodesign and DNA engineeringExplore important ethical issues raised by examples of synthetic biologyInvestigate the BioBuilder labs that probe the design-build-test cycleTest synthetic living systems designed and built by engineersMeasure several variants of an enzyme-generating genetic circuitModel "bacterial photography" that changes a strain’s light sensitivityBuild living systems to produce purple or green pigmentOptimize baker’s yeast to produce ?-carotene
Each chapter of the book investigates a facet of the physical world, including the drag on small projectiles; the importance of diffusion and convection; the size-dependence of acceleration; the storage, conduction, and dissipation of heat; the relationship among pressure, flow, and choice in biological pumps; and how elongate structures tune their relative twistiness and bendiness. Vogel considers design-determining factors all too commonly ignored, and builds a bridge between the world described by physics books and the reality experienced by all creatures. Glimpses of Creatures in Their Physical Worlds contains a wealth of accessible information related to functional biology, and requires little more than a basic background in secondary-school science and mathematics.
Drawing examples from creatures of land, air, and water, the book demonstrates the many uses of biological diversity and how physical forces impact biological organisms.
This book provides a roadmap to the broad and varied career development opportunities in bioengineering, biotechnology, and related fields. Eminent practitioners lay out career paths related to academia, industry, government and regulatory affairs, healthcare, law, marketing, entrepreneurship, and more.
Lifetimes of experience and wisdom are shared, including "war stories," strategies for success, avoidance of common pitfalls, and discussions of the authors’ personal views and motivations. Career Development in Bioengineering and Biotechnology is an indispensable guide to some of the most exciting career and professional growth opportunities in science, engineering, and beyond, and a "must read" for anyone interested in a career related to this burgeoning field.
From the Foreword by U.S. National Medal of Science Laureate and Institute Professor Robert Langer, Massachusetts Institute of Technology: "This book provides a wealth of information and should serve as an excellent resource...The editors have gone to great effort to discuss a variety of critical topics in the burgeoning areas of bioengineering and biotechnology."
From the Introduction by Dr. Bruce Alberts, President Emeritus of the U.S. National Academy of Sciences and Co-chair of the InterAcademy Council: "I am very impressed with the enormous dedication and skill that created this major, highly-original contribution – I know of nothing like it."
From the Editorial by Dr. Joachim Nagel, President, International Union for Physical and Engineering Sciences in Medicine, and past president of the International Federation for Medical and Biological Engineering: "This book provides all the answers and can be highly recommended as the ultimate guide to anyone interested in bioengineering and biotechnology. The book arrives at a crucial time, and catapults bioengineering and biotechnology to the forefront of disciplines and to a rightly held pinnacle of inspiration for engineers, scientists, and technologists."
From the Afterword by Dr. Shu Chien, President, Biomedical Engineering Society and past president of the American Physiological Society and of the American Institute of Medical and Biological Engineering: "...this is truly an outstanding book that is the first of its kind...certainly a pioneering contribution."
Praise for the Book
"Bioengineering and Biotechnology are emerging as distinct disciplines amid the biological revolution and during a period of rapid globalization. These interesting times offer us unprecedented opportunities for professional and personal growth. This book covers many important areas of opportunity, including entrepreneurship, finance, law, and education, with a global perspective. The legacy of our times will include how well we used our rapidly advancing technologies to improve the world around us. This book provides a roadmap for the contributions of Bioengineering and Biotechnology in this quest."
-James E. Moore, PhD, Texas A&M University
"This book will be essential reading for all those seeking career guidance in bioengineering and biotechnology."
-Tony Bradshaw, PhD, Director bioProcessUK - BioIndustry Association (BIA), Chairman, The Royal Academy of Engineering/BIA Life Scientists' Career Seminars
"...the topics [are] quite extensive covering definitions, core curriculum, career opportunities, including a wide range of alternative career pathways as well as social and ethical issues. The material covered is unlike any of the standard publications related to these fields of activity... [the book] can be read at different stages of one's career."
-Joseph D. Bronzino, PhD, Trinity College
"...once I started reading it, [I] could not put it down. In less than three days, I read it all, absorbing the stories and details as if I was consummed by watching a high action movie... The breath and depth of the wisdom is phenomenal, and the stories shared by the writers are moving, inspiring, and shine of intelligence in seizing one's own passion and talents and turning them into stellar professional careers."
-Nathalie Gosset, MS, MBA, Head of Marketing, Alfred Mann Institute for Biomedical Engineering, University of Southern California
"This is a functional book with immediate impact, and is very helpful to those who need and desperately want help in making a career choice."
- Jonathan Newman, Graduate Student in Biomedical Engineering, Georgia Institute of Technology, USA
"This is an exciting undertaking and very well thought through and balanced. I enjoyed very much reading the chapters I have reviewed. Congratulations to all contributors and the editors of this book."
- Gudrun Zahlmann, PhD, Director of Business Development, Siemens Medical Systems, Germany
"I am very excited about this book. As a bioengineering educator, I am always looking for information that can provide guidance for students as they prepare for their careers. The contributors in this book are so enthusiastic about their careers that many of the chapters made me want to switch careers on the spot! I believe that engineering students do not receive enough guidance on alternative career paths. This book will very much help fill the void."
- Judy Cezeaux, PhD, Professor of Biomedical Engineering, Western New England College, Massachusetts, USA
The volume is intended for graduate-level biology students as well as researchers who wish to gain a better understanding of applied bioinformatics and who wish to use bioinformatics technologies to assist in their research. The volume would also be of value to bioinformatics developers, particularly those from a computing background, who would like to understand the application of computational tools for biological research. Each chapter would include a comprehensive introduction giving an overview of the fundamentals, aimed at introducing graduate students and researchers from diverse backgrounds to the field and bring them up-to-date on the current state of knowledge. To accommodate the broad range of topics in applied bioinformatics, chapters have been grouped into themes: gene and genome analysis, molecular genetic analysis, gene expression analysis, protein and proteome analysis, metabolome analysis, phenome data analysis, literature mining and bioinformatics tool development. Each chapter and theme provides an introduction to the biology behind the data describes the requirements for data processing and details some of the methods applied to the data to enhance biological understanding.
The automobile was once seen as a boon to American life, eradicating the pollution caused by horses and granting citizens new levels of personal freedom and mobility. But it was not long before the servant became the master—public spaces were designed to accommodate the automobile at the expense of the pedestrian, mass transportation was neglected, and the poor, unable to afford cars, saw their access to jobs and amenities worsen. Now even drivers themselves suffer, as cars choke the highways and pollution and congestion have replaced the fresh air of the open road.
Today our world revolves around the car—as a nation, we spend eight billion hours a year stuck in traffic. In Asphalt Nation, Jane Holtz Kay effectively calls for a revolution to reverse our automobile-dependency. Citing successful efforts in places from Portland, Maine, to Portland, Oregon, Kay shows us that radical change is not impossible by any means. She demonstrates that there are economic, political, architectural, and personal solutions that can steer us out of the mess. Asphalt Nation is essential reading for everyone interested in the history of our relationship with the car, and in the prospect of returning to a world of human mobility.
Erik Seedhouse is eminently qualified to describe the effects of large accelerations on the body. In addition to being the author of several previously published Springer Praxis books, he has developed astronaut-training protocols and is the training director for Astronauts for Hire (A4H). He is also the Canadian Forces’ High Risk Acceleration Training Officer.
Rather than being a side-by-side collection of articles, this book consists of related chapters. It is a common achievement by 76 experts from around the world. Their expertise reflects the diversity of the field with radiation therapy, medical and accelerator physics, radiobiology, computer science, engineering, and health economics. The book addresses a similarly broad audience ranging from professionals that need to know more about this novel treatment modality or consider to enter the field of ion beam therapy as a researcher. However, it is also written for the interested public and for patients who might want to learn about this treatment option.
Each chapter includes a discussion of recent technological developments and the biological effects of the imaging modality. End-of-chapter problem sets, lists of relevant references, and suggested further reading are presented for each technique.X-ray imaging, including CT and digital radiographyRadionuclide imaging, including SPECT and PETUltrasound imagingMagnetic resonance imaging
In biomedical research, because of a dramatic increase in productivity, immunocytochemistry has emerged as a major technique. The proposed book will provide the first practical guide to planning, performing, and evaluating immunocytochemical experiments.
In today’s graduate education the emphasis is on doing research and not on formal class work. Graduate students therefore lack the background in many essential techniques necessary to perform research in fields in which they were not trained. As director of a university core microscopy facility which sees students and faculty from dozens of laboratories each year, Dr. Burry has surmised the vast majority of these novice microscope users need considerable help. In an attempt to educate users, Dr. Burry has initiated immunocytochemistry seminars and workshops which serve to train people in this powerful research tool. The proposed book is an outgrowth of these presentations and conversations with, by now, hundreds of people who have asked for help.
The philosophy which separates this book from other books in this field is that it is practical, rather than academic. In looking at other important immunocytochemistry titles, the predominant orientation is academic, with the author attempting to comprehensively discuss the topic. For example, one book with sample preparation lists ten fixatives which can be used; however, only two such fixatives are commonly used today. In this particular title, the detailed discussion of old methods might be seen as important in establishing the author as an expert. By contrast, the approach for Burry’s book would be to discuss methods based on what works in animal research laboratories today, and focus only on the most productive methods.
An additional distinction with this proposed book is the focus on animal research and not human pathology. There is a certification program for pathology technicians which requires them to learn a set body of material based on processing human tissue for examination by a pathologist. Many of the books on immunocytochemistry aim at this large pathology user base. Due to historical reasons, pathology laboratories process human tissues in a specific way and embed the tissue in paraffin, as has been done for over a century. In the last ten years, the power of immunocytochemistry in clinical diagnosis has become clear and has accordingly been adapted to pathology. However, the extensive processing needed for paraffin sections is not needed if the tissues are from research animals. Processing for animal-based tissues takes about a third of the time and results in higher quality images. The focus of this book is on processing these animal research tissues for immunocytochemistry. Today, there are no technique books which are aimed at this user base.
As a subject matter expert in the area of the proposed book, Dr. Burry will make recommendations and offer opinions. Because this field is new and is emerging, there are numerous advantages of specific methods over other, more generalized methods. The purpose of this book is to show a novice how to do immunocytochemistry without engaging in a discussion of possible advanced methods. For the advanced user, there are several good books which discuss the unusual methods, yet for the novice there are currently none.
Main Author :
Richard W. Burry, The Ohio State University (United States).
The Outline of the Book :
Each chapter supplies a set of important principals and steps necessary for good immunocytochemistry. The information is distilled down to include only the most important points and does not attempt to cover infrequently used procedures or reagents. At the end of most chapters is a section on trouble-shooting many of the common problems using the Sherlock Holmes method. Each chapter also includes specific protocols which can be used. The goal of each chapter is to present the reader with enough information to successfully design experiments and solve many of the problems one may encounter. Using immunocytochemical protocols without the understanding of their workings is not advised, as the user will need to evaluate his or her results to determine whether the results are reliable. Such evaluation is extremely important for users who need reliable images which will clearly answer important scientific questions.
Definitions (immunocytochemistry and immunohistochemistry)
Scope: animal research and not human pathology, paraffin sections, epitope retrieval, or immunohistochemistry
Focus: fluorescence and enzyme detection
Why do immunocytochemistry?
Immunocytochemistry "individual study" rather than "population study"
Example of a two-label experiment
What is included in these chapters?
Overview of the theory
Background with enough information to help solve common problems.
Advantages and disadvantages of different options
Opinions and suggestions
2. Fixation and Sectioning
Chemistry of fixation
Denaturing vs cross-linking fixatives
Application of fixative
Perfusion, drop-in, cultures, fresh-frozen
Selection of sample section type
Rapid freezing, cryostat, freezing microtome, vibratome
Storage of tissue
Isoforms, structure, reactivity
Polyclonal vs monoclonal
Antibodies as reagents
Antibody specificity and sources
Storage and handling
4. Labels for antibodies
Fluorescence, enzymes and particulates
Fluorescent labels - four generations
Selecting enzymes vs. fluorescence
Selecting a label- advantages and disadvantages
5. Methods of applying antibodies
Antibody amplification methods
6. Blocking and Permeability
Theory of blocking
Theory of detergents
7. Procedure- Single primary antibody
Sample, fixation, sectioning
8. Multiple primary antibodies - primary antibodies of different species
9. Multiple primary antibodies-primary antibodies of same species
Wide-field fluorescence microscope
Bright field—enzyme chromogen
Size, intensity, and pixels
Manipulation—what is ethical?
11. Planning and Troubleshooting
Scheme for discussion-making in planning experiments
Case studies with Sherlock Holmes detective work
12. So you want to do electron microscopic ICC?
Criteria in decision-making
Summary of the two techniques
A radical, how-to guide for using exponential technologies, moonshot thinking, and crowd-powered tools, Bold unfolds in three parts. Part One focuses on the exponential technologies that are disrupting today’s Fortune 500 companies and enabling upstart entrepreneurs to go from “I’ve got an idea” to “I run a billion-dollar company” far faster than ever before. The authors provide exceptional insight into the power of 3D printing, artificial intelligence, robotics, networks and sensors, and synthetic biology. Part Two draws on insights from billionaires such as Larry Page, Elon Musk, Richard Branson, and Jeff Bezos and reveals their entrepreneurial secrets. Finally, Bold closes with a look at the best practices that allow anyone to leverage today’s hyper-connected crowd like never before. Here, the authors teach how to design and use incentive competitions, launch million-dollar crowdfunding campaigns to tap into tens of billions of dollars of capital, and finally how to build communities—armies of exponentially enabled individuals willing and able to help today’s entrepreneurs make their boldest dreams come true.
-Mendel Kleiner, author of Worship Space Acoustics, Acoustics: Information and Communication Series (J. Ross Publishing 2010)
“All involved in their design will appreciate this presentation of recent rooms for religious worship.”
-Leo L. Beranek, author of Concert Halls and Opera Houses: Music, Acoustics, and Architecture (Springer-Verlag 2004)
“Through descriptions, photos, drawings, and acoustical data, this book provides valuable information on existing worship spaces designed during the past thirty years. This very well-edited book, including the Editors' Preface and six excellent essays from key people involved in worship space design, provides valuable information and ideas on the aesthetic, acoustic, and liturgical design of worship spaces for a number of faiths and in several countries.”
-Robert Coffeen, principle at R. C. Coffeen, Consultant in Acoustics LLC, Lawrence, Kansas
This book takes the reader on a wide-ranging tour through churches, synagogues, mosques, and other worship spaces designed during the past 30 years. The bookbegins with a series of essays on topics ranging from the soundscape of worship spaces to ecclesiastical design at the turn of the 21st Century. Perspective pieces from an architect, audio designer, music director, and worship space owner are also included. The core of the book presents the acoustical and architectural design of a wide variety of individual worship space venues. Acoustical consulting firms, architects, and worship space designers from across the world contributed their recent innovative works in the area of worship space acoustics. The contributions include detailed renderings and architectural drawings, as well as informative acoustic data graphs and evocative descriptions of the spaces. Filled with beautiful photography and fascinating modern design, this book is a must-read for anyone interested in religious architecture, acoustical design, or musical performance.
Intended for science and engineering students with one year of introductory physics background, this textbook presents the medical applications of fundamental principles of physics to students who are considering careers in medical physics, biophysics, medicine, or nuclear engineering. It also serves as an excellent reference for advanced students, as well as medical and health researchers, practitioners, and technicians who are interested in developing the background required to understand the changing landscape of medical science. Practice exercises are included and solutions are available separately in an instructor's manual.Complete discussion of the fundamental physical principles underlying modern medicineAccessible exploration of the physics encountered in a typical visit to a doctorPractice exercises are included and solutions are provided in a separate instructor’s manual (available to professors)A companion website (modernphysicsinmedicine.com) presents supplementary materials