The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes.Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineersExplains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrologyDiscusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculationsConsiders the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applicationsDiscusses the theory and design of near-field optical microscopesExplains phase imaging in the scanning optical and interference microscopes
This book offers a number of new results from well-known authors who are engaged in aspects of the development of novel physical principles, new methods, or implementation of modern technological solutions into current imaging devices and new applications of high-resolution imaging systems. The ultimate purpose of this book is to encourage more research and development in the field to realize the great potential of high resolution acoustic imaging and its various industrial and biomedical applications.
The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications.Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications.
Tips/advice on building systems and working with advanced imaging biomedical techniques, including interpretation of phase images, and techniques for quantitative analysis based on phase microscopy.
Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples.