With its wide range of topics and long historical pedigree, Advances in Enzymology and Related Areas of Molecular Biology can be used not only by students and researchers in molecular biology, biochemistry, and enzymology, but also by any scientist interested in the discovery of an enzyme, its properties, and its applications.
This book outlines first the quantitative procedures and various methods suitable for the determination of amino acids found as constituents of naturally occurring peptides and as free amino acids in tissues and body fluids. These topics are followed by a discussion on some of the aspects of peptide chemistry, which appear significant in relation to peptides possessing physiological activity. The next chapter considers protein synthesis that represents the sequences of chemical reactions whereby amino acids are assembled in biological systems to produce proteins. This volume also examines the correlation of structure with function; the mechanisms of control of protein biosynthesis; the exact role of
intramolecular interactions in the determination of tertiary structure; and the colinearity of genetic “maps with amino acid sequences. A chapter describes the methods of analysis and reactions of sulfhydryl, disulfide, and thiol ester groups in proteins, as well as the evidence relating to the functions of these sulfur groups in proteins. The final chapter looks into the models and theories for the noncovalent bond interactions in proteins.
This book is of value to organic chemists, biochemists, and researchers in the protein-related fields.
The publication first elaborates on biochemistry and geochemistry, water and its biological significance, and the problems of protein structure. Discussions focus on the number of peptide chains in the molecule and nature of terminal groups, latent heat of fusion, characteristics of the amino acids derived from proteins, expansion of water in freezing, and the relative abundance of chemical elements in the universe. The text then takes a look at thermodynamics and the application to polar molecules and ionic solutions of electrostatics, including free energy of a charged sphere, image charges, salting-out effect, expressions for the change of fundamental thermodynamic functions, and chemical potentials.
The book examines the conductivity of electrolytes, acid-base equilibria, and polybasic acids, bases, and ampholytes, including proteins. Topics include ionization of cysteine, isoelectric points of polyvalent ampholytes, hemoglobin, nature of acids and bases, measurement of conductivity, electrolytes as conductors, and the moving boundary method of determining transference numbers.
The manuscript is a dependable reference for chemists and researchers interested in thermodynamics, electrostatics, and the biological value of the properties of matter.