All chapters have been updated with new information and many new tables have been added to elucidate key points. The book now offers discussion on how to handle missing data when analyzing results, and coverage of Adaptive Designs and Effectiveness Designs and new sections on Comparative Effectiveness Research and Pragmatic Trials. Chapter 6 includes new material on Phase 0 Trials, expanded coverage of Futility Trials, a discussion of Medical Device approval, Off Label Drug use and the role of the FDA in regulating advertising. Additional new information includes the role of pill color and shape in association with the placebo effect and an examination of issues surrounding minority recruitment. The final chapter offers a new section on manuscript preparation along with a discussion of various guidelines being adopted by journals: CONSORT, STROBE, PRISMA, MOOSE and others; and coverage of Conflicts of Interest, Authorship, Coercive Citation, and Disclosures in Industry-Related Associations.
Building on the strengths of its predecessor in its comprehensive approach and authoritative advice, the new edition offers more of what has made this book a popular, trusted resource for students and working researchers alike.
The manual separates the laboratory course into two separate modules. The first module familiarizes students with the organism and lab equipment by performing growth curves, Lowry protein assays, quantifying prodigiosin and ATP production, and by performing complementation studies to understand the biochemical pathway responsible for prodigiosin production. Students learn to use Microsoft Excel to prepare and present data in graphical format, and how to calculate their data into meaningful numbers that can be compared across experiments. The second module requires that the students employ UV mutagenesis to generate hyper-pigmented mutants of S. marcescens for further characterization. Students use experimental data and protocols learned in the first module to help them develop their own hypotheses, experimental protocols, and to analyze their own data.
Before each lab, students are required to answer questions designed to probe their understanding of required pre-laboratory reading materials. Questions also guide the students through the development of hypotheses and predictions. Following each laboratory, students then answer a series of post-laboratory questions to guide them through the presentation and analysis of their data, and how to place their data into the context of primary literature. Students are also asked to review their initial hypotheses and predictions to determine if their conclusions are supportive. A formal laboratory report is also to be completed after each module, in a format similar to that of primary scientific literature.
The Fundamentals of Scientific Research: An Introductory Laboratory Manual is an invaluable resource to undergraduates majoring in the life sciences.