An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM).Includes detailed protocols accessible to new usersDetails how to set up and run a "Confocal Microscope Core Facility"Contains over 170 figures
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Addressing readers curious to know why and how he achieved such success, the story begins with a prologue describing the end of World War II and Inoué’s lifelong collaborator, the great cell biologist Katsuma Dan. Following the prologue, the author’s childhood and teenage experiences during World War II are described, before the focus shifts to his scientific career and personal life.
The book not only offers important tips for young researchers, it will also help them develop a passion for science.
Contents: Fluorescence Lifetime Imaging by Double Pulse Excitation in Bilateral Confocal Microscopy (G J Brakenhoff et al.)Icosahedral Virus Structure Determination by Electron Cryomicroscopy: Image Processing Principles (P A Thuman-Commike & W Chiu)Appropriate Image Processing for Confocal Microscopy (G Cox & C J R Sheppard)X-Ray Fluorescence Two-Dimensional Microanalysis at the VEPP-3 Storage Ring: Applications in Environmental Science (I P Dolbnya & K V Zolotarev)3D Transfer-Function Analysis for 4Pi Confocal Microscopy (M Gu & C J R Sheppard)Image Restoration of Thick Biological Specimens for Transmission Electron Microscope Tomography (K F Han et al.)Images of Membrane-Bound Detoxification Enzyme at 4Å Resolution Obtained by Electron Cryomicroscopy (I Schmidt-Krey et al.)The Uptake Pathway of DNA and Lipids in Cationic Liposome-Mediated Gene Transfer (S-W Hui et al.)Multiscale Imaging, Analysis and Modeling of the Pulmonary Bronchial Tree (A Kriete et al.)Asymmetric Cell Division as a Mechanism of Cell Determination in Vascular Plants (B-L Lin)3D Electron Microscopy of Cells and Organelles by High-Voltage EM, Tomography and Stereopair Analysis (M Marko)Surface Scattering Techniques in Scanning Optical Microscopy (J F Aguilar & E R Méndez)Applications of Electron Microscopy to Materials and Earth Sciences – Dislocation, Shape and Orientation Changes and Interdiffusion (P Shen)Surface Profiling and Confocal Microscopy (C J R Sheppard & J C Quartel)Matched Spatial Filters in Long Working Distance Microscopy of Phase Objects (J R Strickler & J-S Hwang)Transport Pathways of Lipoproteins Across the Arterial Endothelial Cells (C-H Kao et al.)Polarisation Effects in Confocal Microscopy (T Wilson)Near-Field Microscopy and Spectroscopy of Single Molecules, Single Proteins and Biological Membranes (X S Xie et al.)
Contents: Multiple Oblique Illumination Method of High Definition Stereo Microscopy (G L Greenberg & A Boyde)Two-Color Confocal Fluorescence Microscopy with Improved Channel Separation: Applications in Chemical Neuroanatomy (K Carlsson & B Ulfhake)Morphological Parameters, Medial Lines and Skeletons Computed in the 3D Voronoi Diagram with Applications to 3D CLSM Images (R Eils et al.)Confocal Imaging Through Weakly and Highly Scattering Media with Ultrashort Pulsed Laser Beams (M Gu et al.)Realisation of Depletion by Stimulated Emission in Fluorescence Microscopy (S W Hell et al.)Cytoskeletal and Nuclear Behavior During Female Gametophyte Development and Fertilization in Angiosperms (B-Q Huang et al.)3D X-Ray Microscopy: High-Resolution Stereo-Imaging with the Göttingen X-Ray Microscope at BESSY (J Lehr)Distribution of Taste Pores and Ultrastructural Organization of Gustatory Cells in Gerbil Vallate Taste Buds (K-S Lu et al.)Triple-Colour Confocal Microscopy: A Tool for the Analysis of Three-Dimensional Biological Structures (E M M Manders et al.)Contrast, Resolution, Bleaching and Statistics in Confocal Microscopy (J Pawley)Membrane Specialization in Skeletal Muscle Cells (H B Peng)The Optical Fractionator (S Antunes et al.)Optical Fibre Probe Microscopy (C J R Sheppard & H Fatemi)The Fractional Fourier and Special Affine Fourier Transformations (S Abe & J T Sheridan)Confocal Theta Fluorescence Microscopy: Practical Considerations (S Lindek & E H K Stelzer)Subcellular Localization and Gene Mapping of Human Nuclear Mitotic Apparatus Protein (NuMA) by Confocal Fluorescence Microscopy (C-J C Tang & T K Tang)Exact and Approximate Cone-Beam X-Ray Microtomography (G Wang et al.)Advantages and Application of Low Temperature Scanning Electron Microscopy (W P Wergin et al.)Sensitivity of Optical Near-Field to a Two-Dimensional Rectangular Structure (S Wang)