CRISPR-Cas system based diagnosis of infectious diseases

· ROOHI BANSAL
4.5
4 reviews
Ebook
214
Pages

About this ebook

Since its discovery as a part of the bacterial defense mechanism, the Nobel Prize-winning technology CRISPR-Cas system has revolutionized the fields of genome editing and genetic engineering. Beyond gene-editing ability, scientists have leveraged its potential in the diagnosis of infectious diseases including COVID-19.

This book provides a detailed understanding of CRISPR-based rapid and point-of-care diagnostic kits like SHERLOCK, DETECTR, FELUDA, AIOD CRISPR-Cas12a, etc. Although these CRISPR-based tests are performed using isothermal nucleic acid amplification processes like RPA and LAMP, they promise a real-time RT-PCR sensitivity. Furthermore, the tests’ results can be interpreted using paper-based lateral flow strips, potentially reducing laboratory and test costs. In this technique, the colored lines on the strip, similar to pregnancy tests, indicate whether the test is positive or negative. Because of the ease of performing the test and simple interpretation of the test results, CRISPR-based tests can be used at airports, ports, clinics, schools, etc., for better disease diagnosis, monitoring, management, and containment of infectious diseases like COVID-19.

Additionally, the book also discusses Monoclonal Antibodies, which have revolutionized the treatment for cancer, arthritis, autoimmune diseases, etc. This book also talks about various strategies to isolate monoclonal antibodies from the COVID-19 recovered people and different ways to engineer these antibodies using hybridoma technology. 


Ratings and reviews

4.5
4 reviews

About the author

Roohi Bansal has a PhD in Molecular Biology. Her research studies are published in reputed journals like Molecular Microbiology, Journal of Biological Chemistry, etc. Her passion is to teach, and till now, she has taught more than 6000 students from 100+ countries. She also is the founder of Techskill Brew, an online platform, where she creates educational content in the form of videos and blog posts.

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