This unique reference makes some of the esoteric aspects of this important area of analysis more readily comprehensible to those who deal with analytical instruments but who have not been trained in mass spectrometry. It also serves as a refresher for practicing mass spectroscopists by clarifying principles to afford a better appreciation and application of this technology.
Introduction to Protein Mass Spectrometryprovides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products.
This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources.
Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities.Offers introductory information for scientists and researchers new to the field, as well as advanced insight into the critical assessment of computer-analyzed mass spectrometric results and their current limitationsProvides examples of commonly-used MS instruments from Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, and WatersIncludes biological applications and exploration of analytical tools and databases for bioinformatics
It is a definite must-have work for:
- libraries at academic institutions with Master and Graduate programs in biochemistry, molecular biology, structural biology and proteomics
- individual laboratories with interests covering these areas
- libraries and individual laboratories in the pharmaceutical and biotechnology industries.
*Serves as an essential reference to those working in the field
*Incorporates the contributions of prominent experts
*Features comprehensive coverage and a logical structure
This broad textbook in inorganic mass spectrometry, presents the most important mass spectrometric techniques used in all fields of analytical chemistry. By covering recent developments and advances in all fields of inorganic mass spectrometry, this text provides researchers and students with information to answer any questions on this topic as well as providing the basic fundamentals for understanding this potentially complex, but increasingly relevant subject.
Mass spectrometry (MS) is playing an increasingly important role in the study of protein interactions. Mass Spectrometry of Protein Interactionspresents timely and definitive discussions of the diverse range of approaches for studying protein interactions by mass spectrometry with an extensive set of references to the primary literature. Each chapter is written by authors or teams of authors who are international authorities in their fields. This leading reference text:
Discusses the direct detection of protein interactions through electrospray ionization (ESI-MS); ion mobility analysis; and matrix-assisted laser desorption/ionization (MALDI-MS)
Covers the indirect analysis of protein interactions through hydrogen-deuterium exchange (HX-MS); limited proteolysis; cross-linking; and radial probe (RP-MS)
Guides researchers in the use of mass spectrometry in structural biology, biochemistry, and protein science to map and define the huge number and diversity of protein interactions
Reviews the latest discoveries and applications and addresses new and ongoing challenges
This is a comprehensive reference for researchers in academia and industry engaged in studies of protein interactions and an excellent text for graduate and postgraduate students.
The relatively new and rapidly changing field of redox proteomics has the potential to revolutionize how we diagnose disease, assess risks, determine prognoses, and target therapeutic strategies for people with inflammatory and aging-associated diseases. This collection brings together, in one comprehensive volume, a broad array of information and insights into normal and altered physiology, molecular mechanisms of disease states, and new applications of the rapidly evolving techniques of proteomics.
Written by some of the finest investigators in this area, Redox Proteomics: From Protein Modifications to Cellular Dysfunction and Diseases examines the key topics of redox proteomics and redox control of cellular function, including:
* The role of oxidized proteins in various disorders
* Pioneering studies on the development of redox proteomics
* Analytical methodologies for identification and structural characterization of proteins affected by oxidative/nitrosative modifications
* The response and regulation of protein oxidation in different cell types
* The pathological implications of protein oxidation for conditions, including asthma, cardiovascular disease, diabetes, preeclampsia, and Alzheimer's disease
Distinguished by its in-depth discussions, balanced methodological approach, and emphasis on medical applications and diagnosis development, Redox Proteomics is a rich resource for all professionals with an interest in proteomics, cellular physiology and its alterations in disease states, and related fields.