1. OBJECTIVE
In this experiment, you will determine the hydrogen potential of orange juice. To do this, you must initially calibrate a pH meter with buffer solutions of pH 4 and 7. After calibration, you will be able to perform pH measurements with greater precision.
At the end of this experiment, you should be able to:
use a pH meter to measure pH;
calibrate a pH meter;
determine the pH of orange juice.
2. Where to use these concepts?
Measuring the pH of a substance or compound has applications in the most varied areas of our society. For example, in the food sector, measuring and controlling the pH of a product can be essential to prevent the proliferation of bacteria and agents harmful to the human body. In the chemical industry, pH can affect the result of a chemical reaction and, therefore, contribute to greater or lesser production efficiency.
3. The experiment
This experiment will use a pH meter and buffer solutions with pH 4 and 7. These solutions are used to calibrate the pH meter and characterize respectively an acidic and a neutral substance. After this initial calibration, you will determine the pH of the orange juice by immersing the pH meter directly in the juice. The procedure will be carried out in triplicate so that an average can be calculated.
4. Security
In this practice, a lab coat, gloves and glasses will be used. Additionally, you must come to the laboratory wearing long pants and sneakers.
5. Scenario
The laboratory environment has a pH meter (equipment for determining the pH of substances), buffer solutions (solutions used to calibrate the pH meter) and orange juice for analysis.