The seeds morphometry in the series of measurements is performed by following steps: 1) Parameters setup (‘Settings’ option in the main menu); 2) Calibration step (‘Calibration’ option in the main menu); 3) Measurement step (‘Counting’ option in the main menu); 4) Viewing results (‘Seed data’ option in the main menu).
Settings Using this option the user can define the size of the paper sheet (including user-defined sizes), camera and image resolutions and change the touching seed separation algorithm type (watershed or HSV binarization).
Calibration. Calibration needed to adjust algorithm parameters for robust paper and seed boundaries determination. To calibrate the program: Place single grain in the center of the white paper. Select “Calibration” option in the main menu. Obtain the paper image by touching the screen of the mobile device and wait for the recognition result. Make sure that the grain was correctly detected by the algorithm. Repeat the calibration if necessary. The algorithm parameters will be saved automatically.
Measurement of seeds To perform measurement step: Place grains arbitrarily on a sheet of paper. We recommend minimizing any contacts between grains. Select “Counting” option in the main menu. Obtain the paper image by touching the screen of the mobile device and wait for the recognition result. If the paper sheet was not recognized (the warning message appeared), repeat this procedure again. Touch the screen of the mobile device to save result in the memory. Enter the name of the measurement result in the save dialog. To save the results and return to the main menu, select “Save and Exit”. To continue the series of measurements, click “Save and continue”. To return without saving the result, select “Cancel”.
Viewing data To view saved data, select “Seed Data” option in the main menu. Scroll up and down to view the details of the current measurement. Scroll left and right to view other measurements. To clear the list of measurement select "Clean" in the menu. To export data in TSV format select "Export to TSV" in the menu and select measurements for export. You can also to select all measurements for export. To save all the data click on the button "Export".