the drama of the front lines.”
-Richard Danzig, former secretary of the navy
The first major bioterror event in the United States-the anthrax attacks in October 2001-was a clarion call for scientists who work with “hot” agents to find ways of protecting civilian populations against biological weapons. In The Demon in the Freezer, his first nonfiction book since The Hot Zone, a #1 New York Times bestseller, Richard Preston takes us into the heart of Usamriid, the United States Army Medical Research Institute of Infectious Diseases at Fort Detrick, Maryland, once the headquarters of the U.S. biological weapons program and now the epicenter of national biodefense.
Peter Jahrling, the top scientist at Usamriid, a wry virologist who cut his teeth on Ebola, one of the world’s most lethal emerging viruses, has ORCON security clearance that gives him access to top secret information on bioweapons. His most urgent priority is to develop a drug that will take on smallpox-and win. Eradicated from the planet in 1979 in one of the great triumphs of modern science, the smallpox virus now resides, officially, in only two high-security freezers-at the Centers for Disease Control in Atlanta and in Siberia, at a Russian virology institute called Vector. But the demon in the freezer has been set loose. It is almost certain that illegal stocks are in the possession of hostile states, including Iraq and North Korea. Jahrling is haunted by the thought that biologists in secret labs are using genetic engineering to create a new superpox virus, a smallpox resistant to all vaccines.
Usamriid went into a state of Delta Alert on September 11 and activated its emergency response teams when the first anthrax letters were opened in New York and Washington, D.C. Preston reports, in unprecedented detail, on the government’s response to the attacks and takes us into the ongoing FBI investigation. His story is based on interviews with top-level FBI agents and with Dr. Steven Hatfill.
Jahrling is leading a team of scientists doing controversial experiments with live smallpox virus at CDC. Preston takes us into the lab where Jahrling is reawakening smallpox and explains, with cool and devastating precision, what may be at stake if his last bold experiment fails.
Of all the extraordinary and obscure plants that have been fermented and distilled, a few are dangerous, some are downright bizarre, and one is as ancient as dinosaurs—but each represents a unique cultural contribution to our global drinking traditions and our history.
This fascinating concoction of biology, chemistry, history, etymology, and mixology—with more than fifty drink recipes and growing tips for gardeners—will make you the most popular guest at any cocktail party.
Horowitz introduces the reader to dogs’ perceptual and cognitive abilities and then draws a picture of what it might be like to be a dog. What’s it like to be able to smell not just every bit of open food in the house but also to smell sadness in humans, or even the passage of time? How does a tiny dog manage to play successfully with a Great Dane? What is it like to hear the bodily vibrations of insects or the hum of a fluorescent light? Why must a person on a bicycle be chased? What’s it like to use your mouth as a hand? In short, what is it like for a dog to experience life from two feet off the ground, amidst the smells of the sidewalk, gazing at our ankles or knees?
Inside of a Dog explains these things and much more. The answers can be surprising—once we set aside our natural inclination to anthropomorphize dogs. Inside of a Dog also contains up-to-the-minute research—on dogs’ detection of disease, the secrets of their tails, and their skill at reading our attention—that Horowitz puts into useful context. Although not a formal training guide, Inside of a Dog has practical application for dog lovers interested in understanding why their dogs do what they do. With a light touch and the weight of science behind her, Alexandra Horowitz examines the animal we think we know best but may actually understand the least. This book is as close as you can get to knowing about dogs without being a dog yourself.
There are thousands of working dogs all over the US and beyond with incredible abilities—they can find missing people, detect drugs and bombs, pinpoint unmarked graves of Civil War soldiers, or even find drowning victims more than two hundred feet below the surface of a lake. These abilities may seem magical or mysterious, but author Cat Warren shows the science, the rigorous training, and the skilled handling that underlie these creatures’ amazing abilities.
Cat Warren is a university professor and journalist who had tried everything she could think of to harness her dog Solo’s boundless energy and enthusiasm…until a behavior coach suggested she try training him to be a “working dog.” What started out as a hobby soon became a calling, as Warren was introduced to the hidden universe of dogs who do this essential work and the handlers who train them.
Her dog Solo has a fine nose and knows how to use it, but he’s only one of many astounding dogs in a varied field. Warren interviews cognitive psychologists, historians, medical examiners, epidemiologists, and forensic anthropologists, as well as the breeders, trainers, and handlers who work with and rely on these intelligent and adaptable animals daily. Along the way, Warren discovers story after story that prove the capabilities—as well as the very real limits—of working dogs and their human partners. Clear-eyed and unsentimental, Warren explains why our partnership with working dogs is woven into the fabric of society, and why we keep finding new uses for the wonderful noses of our four-legged friends.
Life is the most extraordinary phenomenon in the known universe; but how did it come to be? Even in an age of cloning and artificial biology, the remarkable truth remains: nobody has ever made anything living entirely out of dead material. Life remains the only way to make life. Are we still missing a vital ingredient in its creation?
Using first-hand experience at the cutting edge of science, Jim Al-Khalili and Johnjoe Macfadden reveal that missing ingredient to be quantum mechanics. Drawing on recent ground-breaking experiments around the world, each chapter in Life on the Edge illustrates one of life's puzzles: How do migrating birds know where to go? How do we really smell the scent of a rose? How do our genes copy themselves with such precision? Life on the Edge accessibly reveals how quantum mechanics can answer these probing questions of the universe.
Guiding the reader through the rapidly unfolding discoveries of the last few years, Al-Khalili and McFadden describe the explosive new field of quantum biology and its potentially revolutionary applications, while offering insights into the biggest puzzle of all: what is life? As they brilliantly demonstrate in these groundbreaking pages, life exists on the quantum edge.
– Winner, Stephen Hawking Medal for Science Communication
Why do you fall in love with one person rather than another? In this fascinating and informative book, Helen Fisher, one of the world's leading experts on romantic love, unlocks the hidden code of desire and attachment. Each of us, it turns out, primarily expresses one of four broad personality types—Explorer, Builder, Director, or Negotiator—and each of these types is governed by different chemical systems in the brain. Driven by this biology, we are attracted to partners who both mirror and complement our own personality type.
Until now the search for love has been blind, but Fisher pulls back the curtain and reveals how we unconsciously go about finding the right match. Drawing on her unique study of 40,000 men and women, she explores each personality type in detail and shows you how to identify your own type. Then she explains why some types match up well, whereas others are problematic. (Note to Explorers: be prepared for a wild ride when you hitch your star to a fellow Explorer!) Ultimately, Fisher's investigation into the complex nature of romance and attachment leads to astonishing new insights into the essence of dating, love, and marriage.
Based on entirely new research—including a detailed questionnaire completed by seven million people in thirty-three countries—Why Him? Why Her? will change your understanding of why you love him (or her) and help you use nature's chemistry to find and keep your life partner.
This edition includes greatly expanded focus on stem cells, including adult and embryonic stem cells and progenitor populations that may soon lead to new tissue engineering therapies for heart disease, diabetes, and a wide variety of other diseases that afflict humanity. This up-to-date coverage of stem cell biology and other emerging technologies is complemented by a series of new chapters on recent clinical experience in applying tissue engineering. The result is a comprehensive textbook that we believe will be useful to students and experts alike.
New to this edition:
*Includes new chapters on biomaterial-protein interactions, nanocomposite and three-dimensional scaffolds, skin substitutes, spinal cord, vision enhancement, and heart valves
*Expanded coverage of adult and embryonic stem cells of the cardiovascular, hematopoietic, musculoskeletal, nervous, and other organ systems
Beginning with simple theoretical models and experimental techniques, the book develops the complete repertoire of theoretical principles and experimental techniques necessary for understanding and implementing the most sophisticated NMR experiments.
Important new techniques and applications of NMR spectroscopy have emerged since the first edition of this extremely successful book was published in 1996. This updated version includes new sections describing measurement and use of residual dipolar coupling constants for structure determination, TROSY and deuterium labeling for application to large macromolecules, and experimental techniques for characterizing conformational dynamics. In addition, the treatments of instrumentation and signal acquisition, field gradients, multidimensional spectroscopy, and structure calculation are updated and enhanced.
The book is written as a graduate-level textbook and will be of interest to biochemists, chemists, biophysicists, and structural biologists who utilize NMR spectroscopy or wish to understand the latest developments in this field.Provides an understanding of the theoretical principles important for biological NMR spectroscopyDemonstrates how to implement, optimize and troubleshoot modern multi-dimensional NMR experimentsAllows for the capability of designing effective experimental protocols for investigations of protein structures and dynamicsIncludes a comprehensive set of example NMR spectra of ubiquitin provides a reference for validation of experimental methods
Interested in unraveling the physics of living things? Here's your starting point. Biophysics Demystified is the fast and easy way to understand this fascinating topic.
Written in a step-by-step format, this practical guide begins with an introduction to the science of biophysics, covering biophysical techniques and applications. Next, you'll learn the principles of physics, biology, and chemistry required to understand biophysics, including free energy, entropy, and statistical mechanics. Biomolecules and the forces that influence their structure and conformation are also covered, as are protein, nucleic acid, and membrane biophysics. Detailed examples and concise explanations make it easy to understand the material, and end-of-chapter quizzes and a final exam help reinforce key concepts.
It's a no-brainer! You'll get:Molecular, subcellular, physiological, anatomical, and environmental biophysics The laws of thermodynamics as they apply to biophysical systems Forces affecting conformation in biological molecules The composition and structure of carbohydrates, lipids, proteins, and nucleic acids The fluid mosaic model
Simple enough for a beginner, but challenging enough for an advanced student, Biophysics Demystified makes this interdisciplinary subject easy to master.
In April 1982, ethnobotanist Wade Davis arrived in Haiti to investigate two documented cases of zombis—people who had reappeared in Haitian society years after they had been officially declared dead and had been buried. Drawn into a netherworld of rituals and celebrations, Davis penetrated the vodoun mystique deeply enough to place zombification in its proper context within vodoun culture. In the course of his investigation, Davis came to realize that the story of vodoun is the history of Haiti—from the African origins of its people to the successful Haitian independence movement, down to the present day, where vodoun culture is, in effect, the government of Haiti’s countryside.
The Serpent and the Rainbow combines anthropological investigation with a remarkable personal adventure to illuminate and finally explain a phenomenon that has long fascinated Americans.
With The Book of Barely Imagined Beings, Caspar Henderson offers readers a fascinating, beautifully produced modern-day menagerie. But whereas medieval bestiaries were often based on folklore and myth, the creatures that abound in Henderson’s book—from the axolotl to the zebrafish—are, with one exception, very much with us, albeit sometimes in depleted numbers. The Book of Barely Imagined Beings transports readers to a world of real creatures that seem as if they should be made up—that are somehow more astonishing than anything we might have imagined. The yeti crab, for example, uses its furry claws to farm the bacteria on which it feeds. The waterbear, meanwhile, is among nature’s “extreme survivors,” able to withstand a week unprotected in outer space. These and other strange and surprising species invite readers to reflect on what we value—or fail to value—and what we might change.
A powerful combination of wit, cutting-edge natural history, and philosophical meditation, The Book of Barely Imagined Beings is an infectious and inspiring celebration of the sheer ingenuity and variety of life in a time of crisis and change.
The Olympics are the world's most prestigious stage for athletic competition. Fans both casual and hardcore tune in religiously every few years to watch as men and women push themselves to the limits of human performance. But what makes a champion? Is it genetics? Hours of training? A psychological advantage? Of all the athletes who dedicate their lives - and bodies - to achieving that perfect moment of triumph, why will one person or team win out over another? Science has some compelling answers, and in this book, The Science of Sports: Winning in the Olympics, Scientific American explores this topic from various angles. Beginning with Section 1: The Psychology of Winning, the book opens with a look inside the mind of an elite athlete and tackles questions of how to face a rivalry or maintain a positive attitude in the face of defeat. Other sections discuss the sticky issues surrounding genetic advantages and physical prowess, drugs and doping, injury and recovery, and - finally - the latest scientific advice for the rest of us mere mortals to be fit and healthy. You'll find both inspiration and answers in this indispensable book from the editors of Scientific American, the leading authority on science, technology and innovation.
• Ants are world-class road builders, handling traffic problems on thoroughfares that dwarf our highway systems in their complexity
• Ants with the largest societies often deploy complicated military tactics
• Some ants have evolved from hunter-gatherers into farmers, domesticating other insects and growing crops for food
*Contains thorough tutorial treatments, coupled with coverage of advanced topics
*Three of the four holders of the prestigious Institute of Electrical and Electronics Engineers Medical Imaging Scientist Award are chapter contributors
*Include color artwork
One of the most diverse of modern scientific disciplines, biophysics applies methods and technologies from physics to the study of biological systems and phenomena, from the human nervous system to soil erosion to global warming. What are the best options for satisfying the world's growing energy demands? How can we feed the world's growing population? How can we contain, or reverse, global warming? How can we vouchsafe a plentiful supply of potable water for future generations? These are among the critical questions to which biophysicists work to provide answers.Biophysics courses are increasingly taken by students of biology, physics, chemistry, biochemistry, physiology, statistics, bioengineering, neuroscience, computer science, pharmacology, agriculture,and many more Provides a friendly, unintimidating overview of the material covered in a typical college-level biophysics course A one-stop reference, course supplement and exam preparation tool for university students currently enrolled in an introductory biophysics courses An indispensable resource for those studying the natural sciences, biological sciences, and physics, as well as math, statistics,computer science, pharmacology and many other disciplines The current job market for people well versed in biophysics is very strong, and biophysics is currently listed as one of the fast-growing occupations in the North America
"The most scientifically important dog in over a century." —Brian Hare
Chaser has fascinated dog lovers and scientists alike. Her story reveals the potential for taking out dialogue with dogs well beyond "fetch." When retired psychology professor John Pilley first got his new Border collie puppy, Chaser, he wanted to explore the boundaries of language learning and communication between humans and man's best friend. Exhibiting intelligence previously thought impossible in dogs, Chaser soon learned the names of more than a thousand toys and sentences with multiple elements of grammar. Chaser's accomplishments are revolutionizing the way we think about the intelligence of animals. John and Chaser's inspiring journey demonstrates the power of learning through play and opens our eyes to the boundless potential in the animals we love.
This new edition discusses biological systems that can be analyzed quantitatively, and how advances in the life sciences have been aided by the knowledge of physical or engineering analysis techniques. The volume is organized into 18 chapters encompassing thermodynamics, electricity, optics, sound, solid mechanics, fluid mechanics, and atomic and nuclear physics. Each chapter provides a brief review of the background physics before focusing on the applications of physics to biology and medicine. Topics range from the role of diffusion in the functioning of cells to the effect of surface tension on the growth of plants in soil and the conduction of impulses along the nervous system. Each section contains problems that explore and expand some of the concepts. The text includes many figures, examples and illustrative problems and appendices which provide convenient access to the most important concepts of mechanics, electricity, and optics in the body.
Physics in Biology and Medicine will be a valuable resource for students and professors of physics, biology, and medicine, as well as for applied health workers.Provides practical techniques for applying knowledge of physics to the study of living systemsPresents material in a straight forward manner requiring very little background in physics or biologyIncludes many figures, examples and illustrative problems and appendices which provide convenient access to the most important concepts of mechanics, electricity, and optics in the body
This concise introductory paperback surveys and relates basic physics to living systems. It discusses biological systems that can be analyzed quantitatively, and how advances in the life sciences have been aided by the knowledge of physical or engineering analysis techniques.
This text is designed for premed students, doctors, nurses, physiologists, or other applied health workers, and other individuals who wish to understand the nature of the mechanics of our bodies.
- Provides practical techniques for applying knowledge of physics to the study of living systems
- Presents material in a straight forward manner requiring very little background in physics or biology
- Includes many figures, examples and illustrative problems and appendices which provide convenient access to the most important concepts of mechanics, electricity, and optics
The aim of The Nutritional Biochemistry of Chromium(III) is to examine the four most controversial areas of chromium nutrition and biochemistry:
- is chromium an essential element for humans and are chromium nutritional supplements of value?
- what biochemical role, if any, does chromium play in the body
- can large doses of chromium(III) be used to treat symptoms of type 2 diabetes, cardiovascular disease, and related medical conditions
- is the use of chromium(III) supplements a health concern.
Scientific experts, who are recognized leaders in the field, weigh in with their opinions on both sides of these issues in this book.
A background review of the field from 1955-1995 by Vincent opens the book and concludes with a summary by Dr. Forrest Nielsen, Center Director of the USDA's Grand Forks Human Nutrition Research Center concludes the book.
* Point-counterpoint format, providing both sides of major issues
* Complete coverage of current issues, including nutrition, health, biochemical role and toxicology
* Authors are recognised experts and leaders in this field
As advances in algorithms and computer technology have bolstered the digital signal processing capabilities of real-time sonar, radar, and non-invasive medical diagnostics systems, cutting-edge military and defense research has established conceptual similarities in these areas. Now civilian enterprises can use government innovations to facilitate optimal functionality of complex real-time systems. Advanced Signal Processing details a cost-efficient generic processing structure that exploits these commonalities to benefit commercial applications.
Learn from a Renowned Defense Scientist, Researcher, and Innovator
The author preserves the mathematical focus and key information from the first edition that provided invaluable coverage of topics including adaptive systems, advanced beamformers, and volume visualization methods in medicine. Integrating the best features of non-linear and conventional algorithms and explaining their application in PC-based architectures, this text contains new data on:
Advances in biometrics, image segmentation, registration, and fusion techniques for 3D/4D ultrasound, CT, and MRI
Fully digital 3D/ (4D: 3D+time) ultrasound system technology, computing architecture requirements, and relevant implementation issues
State-of-the-art non-invasive medical procedures, non-destructive 3D tomography imaging and biometrics, and monitoring of vital signs
Cardiac motion correction in multi-slice X-ray CT imaging
Space-time adaptive processing and detection of targets interference-intense backgrounds comprised of clutter and jamming
With its detailed explanation of adaptive, synthetic-aperture, and fusion-processing schemes with near-instantaneous convergence in 2-D and 3-D sensors (including planar, circular, cylindrical, and spherical arrays), the quality and illustration of this text’s concepts and techniques will make it a favored reference.
The Clinical Engineering Handbook meets a long felt need for a comprehensive book on all aspects of clinical engineering that is a suitable reference in hospitals, classrooms, workshops, and governmental and non-governmental organization. The Handbook’s thirteen sections address the following areas: Clinical Engineering; Models of Clinical Engineering Practice; Technology Management; Safety Education and Training; Design, Manufacture, and Evaluation and Control of Medical Devices; Utilization and Service of Medical Devices; Information Technology; and Professionalism and Ethics. The Clinical Engineering Handbook provides the reader with prospects for the future of clinical engineering as well as guidelines and standards for best practice around the world. From telemedicine and IT issues, to sanitation and disaster planning, it brings together all the important aspects of clinical engineering.Clinical Engineers are the safety and quality faciltators in all medical facilitiesThe most definitive, comprehensive, and up-to-date book available on the subject of clinical engineeringOver 170 contributions by leaders in the field of clinical engineering
In the late spring and early summer, as a bee colony becomes overcrowded, a third of the hive stays behind and rears a new queen, while a swarm of thousands departs with the old queen to produce a daughter colony. Seeley describes how these bees evaluate potential nest sites, advertise their discoveries to one another, engage in open deliberation, choose a final site, and navigate together--as a swirling cloud of bees--to their new home. Seeley investigates how evolution has honed the decision-making methods of honeybees over millions of years, and he considers similarities between the ways that bee swarms and primate brains process information. He concludes that what works well for bees can also work well for people: any decision-making group should consist of individuals with shared interests and mutual respect, a leader's influence should be minimized, debate should be relied upon, diverse solutions should be sought, and the majority should be counted on for a dependable resolution.
An impressive exploration of animal behavior, Honeybee Democracy shows that decision-making groups, whether honeybee or human, can be smarter than even the smartest individuals in them.
Companion resources site
This book is accompanied by a resources site:
The site is being constantly updated by the author team and provides:
· Related videos selected by the authors
· Updates to the book since publication
· Instructor resources
· A chance to send in feedback
Many will be amazed to learn that all these questions, and many more, can be answered by a single point of discussion—mitochondria and bioenergetics. This legendary saga began over two billion years ago, when one bacterium entered another without being digested, ultimately creating the first mitochondrion.
Since then, for life to exist beyond single-celled bacteria, it’s the mitochondria that are responsible for this life-giving energy. Yet, current research has also revealed a dark side; many seemingly unconnected degenerative diseases have their roots in dysfunctional mitochondria. Modern research, however, has also endowed us with the knowledge on how to optimize its function, which is of critical importance to our health and longevity. By reading this book, you are about to dive into this epic story, and learn how to add years to your life, and life to your years.
Covering a wide range of applications, Introduction to Physics in Modern Medicine, Second Edition builds on the bestselling original. Based on a course taught by the author, the book provides medical personnel and students with an exploration of the physics-related applications found in state-of-the-art medical centers.
Requiring no previous acquaintance with physics, biology, or chemistry and keeping mathematics to a minimum, the application-dedicated chapters adhere to simple and self-contained qualitative explanations that make use of examples and illustrations. With an enhanced emphasis on digital imaging and computers in medicine, the text gives readers a fundamental understanding of the practical application of each concept and the basic science behind it.
This book provides medical students with an excellent introduction to how physics is applied in medicine, while also providing students in physics with an introduction to medical physics. Each chapter includes worked examples and a complete list of problems and questions.
That so much of the technology discussed in this book was the stuff of dreams just a few years ago, makes this book as fascinating as it is practical, both for those in medicine as well as those in physics who might one day discover that the project they are working on is basis for the next great medical application.
Covers hybrid scanners for cancer imaging and the interplay of molecular medicine with imaging technologies such as MRI, CT and PET
Looks at camera pills that can film from the inside upon swallowing and advances in robotic surgery devices
Explores Intensity-Modulated Radiation Therapy, proton therapy, and other new forms of cancer treatmentReflects on the use of imaging technologies in developing countries
For the first time, the University of California Press is offering this resource as an e-book. The Digital Jepson Manual provides an unparalleled new level of interactivity, portability, and convenience. Extensive linking and e-book–friendly illustrations make it easier for users to learn about plant characteristics and identify the native and naturalized plants of California—all in a format ideally suited for use in the field. Using readily available e-book readers, field researchers, students, and enthusiasts can click on links to rapidly navigate through keys to families, genera, species, and subspecies or varieties. Specific features of The Digital Jepson Manual include the following:
—Keys link forward and backward to other taxonomic levels.
—Plate references in taxonomic treatments link to plates for rapid reference.
—Plate captions link to taxonomic treatments.
—Individual taxon figures appear next to species descriptions, and full plates are gathered in a special section.
—Glossary terms link to any relevant illustrations.
—List of families links each family to its taxonomic treatment.
—Index is fully linked to taxonomic treatments.
Optimal devices: iPad, Kindle Fire (but only after conversion)
Will also work on: Computers, Barnes and Noble Nook, Kindle (after conversion)
Longlisted for the PEN/E.O. Wilson Literary Science Writing Award
One of Nature's Summer Book Picks
One of Publishers Weekly's Top Ten Spring 2013 Science Books
For centuries, we've toyed with our creature companions, breeding dogs that herd and hunt, housecats that look like tigers, and teacup pigs that fit snugly in our handbags. But what happens when we take animal alteration a step further, engineering a cat that glows green under ultraviolet light or cloning the beloved family Labrador? Science has given us a whole new toolbox for tinkering with life. How are we using it?
In Frankenstein's Cat, the journalist Emily Anthes takes us from petri dish to pet store as she explores how biotechnology is shaping the future of our furry and feathered friends. As she ventures from bucolic barnyards to a "frozen zoo" where scientists are storing DNA from the planet's most exotic creatures, she discovers how we can use cloning to protect endangered species, craft prosthetics to save injured animals, and employ genetic engineering to supply farms with disease-resistant livestock. Along the way, we meet some of the animals that are ushering in this astonishing age of enhancement, including sensor-wearing seals, cyborg beetles, a bionic bulldog, and the world's first cloned cat.
Through her encounters with scientists, conservationists, ethicists, and entrepreneurs, Anthes reveals that while some of our interventions may be trivial (behold: the GloFish), others could improve the lives of many species-including our own. So what does biotechnology really mean for the world's wild things? And what do our brave new beasts tell us about ourselves?
With keen insight and her trademark spunk, Anthes highlights both the peril and the promise of our scientific superpowers, taking us on an adventure into a world where our grandest science fiction fantasies are fast becoming reality.
Developed at MIT in collaboration with award-winning high school teachers, BioBuilder teaches the foundational ideas of the emerging synthetic biology field, as well as key aspects of biological engineering that researchers are exploring in labs throughout the world. These lessons will empower teachers and students to explore and be part of solving persistent real-world challenges.Learn the fundamentals of biodesign and DNA engineeringExplore important ethical issues raised by examples of synthetic biologyInvestigate the BioBuilder labs that probe the design-build-test cycleTest synthetic living systems designed and built by engineersMeasure several variants of an enzyme-generating genetic circuitModel "bacterial photography" that changes a strain’s light sensitivityBuild living systems to produce purple or green pigmentOptimize baker’s yeast to produce ?-carotene
Development and use of robust analytical methods is critical for the ability to generate accurate analytical data. Sample preparation is an integral part of the analytical method. In a survey conducted by LC-GC1, responses indicated that approximately two-thirds of the time spent testing and analyzing samples was spent on the sample preparation portion of the method. In addition, issues related to sample preparation accounted for one-third of the errors generated while performing an analytical method.
A number of challenges exist in the sample preparation/extraction of pharmaceutical dosage forms for potency and purity analysis as well as isolation and identification of impurities and degradants. These challenges increase for complex dosage forms such as some controlled release formulations and other challenging formulations such as suspensions, ointments, transdermal patches, etc. Challenges in developing rapid and rugged sample preparation methods include complete dispersion of the dosage form to facilitate extraction and solubilization of the analytes of interest, dealing with extracted interfering components and addressing drug-excipient interactions. A number of factors must be considered and addressed in each of these areas.
The objective of Sample Preparation of Pharmaceutical Dosage Forms is to provide an overview of the various sample preparation and extraction techniques available for pharmaceutical dosage forms. In addition to the review and discussion of specific techniques, sample preparation method development and trouble shooting strategies are discussed. Multiple examples and case studies are presented to highlight various potential issues and solutions.
Now, in his long-awaited follow-up, Behe presents far more than a challenge to Darwinism: He presents the evidence of the genetics revolution -- the first direct evidence of nature's mutational pathways -- to radically redefine the debate about Darwinism.
How much of life does Darwin's theory explain? Most scientists believe it accounts for everything from the machinery of the cell to the history of life on earth. Darwin's ideas have been applied to law, culture, and politics.
But Darwin's theory has been proven only in one sense: There is little question that all species on earth descended from a common ancestor. Overwhelming anatomical, genetic, and fossil evidence exists for that claim. But the crucial question remains: How did it happen? Darwin's proposed mechanism -- random mutation and natural selection -- has been accepted largely as a matter of faith and deduction or, at best, circumstantial evidence. Only now, thanks to genetics, does science allow us to seek direct evidence. The genomes of many organisms have been sequenced, and the machinery of the cell has been analyzed in great detail. The evolutionary responses of microorganisms to antibiotics and humans to parasitic infections have been traced over tens of thousands of generations.
As a result, for the first time in history Darwin's theory can be rigorously evaluated. The results are shocking. Although it can explain marginal changes in evolutionary history, random mutation and natural selection explain very little of the basic machinery of life. The "edge" of evolution, a line that defines the border between random and nonrandom mutation, lies very far from where Darwin pointed. Behe argues convincingly that most of the mutations that have defined the history of life on earth have been nonrandom.
Although it will be controversial and stunning, this finding actually fits a general pattern discovered by other branches of science in recent decades: The universe as a whole was fine-tuned for life. From physics to cosmology to chemistry to biology, life on earth stands revealed as depending upon an endless series of unlikely events. The clear conclusion: The universe was designed for life.
Jonathan Balcombe, animal behaviorist and author of the critically acclaimed Pleasurable Kingdom, draws on the latest research, observational studies and personal anecdotes to reveal the full gamut of animal experience—from emotions, to problem solving, to moral judgment. Balcombe challenges the widely held idea that nature is red in tooth and claw, highlighting animal traits we have disregarded until now: their nuanced understanding of social dynamics, their consideration for others, and their strong tendency to avoid violent conflict. Did you know that dogs recognize unfairness and that rats practice random acts of kindness? Did you know that chimpanzees can trounce humans in short-term memory games? Or that fishes distinguish good guys from cheaters, and that birds are susceptible to mood swings such as depression and optimism?
With vivid stories and entertaining anecdotes, Balcombe gives the human pedestal a strong shake while opening the door into the inner lives of the animals themselves.