When mischievous teenaged cousins Bo Duke (Jonathan Bennett) and Luke Duke (Randy Wayne) are arrested, both boys are paroled to the care of their Uncle Jesse (Willie Nelson) in Hazzard, sentenced to a summer of hard work.
It's not long before the Duke boys learn of Boss Hogg's (Chris McDonald) plans to foreclose on Uncle Jesse's farm. Together, with help from their frumpy cousin Daisy (April Scott), Bo and Luke vow to save the family's property and its storied history of producing the best moonshine in all of Hazzard. Driving through Hazzard in the "General Lee" blasting its famed Dixie horn, the Duke boys join forces to make Boss Hogg's life miserable, concocting one crazy scheme after another.