The EzDx-WeD-2 application is developed to connect with the EzDx-WeD-2 device, allowing users to modify its heating curve and display its fluorescence signal in real-time. Here is the user guide for the WeD-2 mobile application: a. Launch the WeD-2 mobile application on the designated smartphone. b. Tap “Bluetooth” to search for available WeD-2 devices within range and connect to the selected WeD-2 device. c. Select the appropriate project, enter relevant information, and tap “Start Detection.”