A Practical Guide to Protein and Peptide Purification for Microsequencing: Edition 2
Paul T. Matsudaira
2012년 12월 · Elsevier
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Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Mass spectrophotometric characterization of proteins and peptides
N-terminal sequencing of proteins with blocked N termini
Internal amino acid sequence analysis after protease digestion in-gel and on-blot
Improved microscale peptide purification methods
Computer analysis of protein sequences
New protocols tested and refined through everyday use in authors' laboratories
Updated reference chapter covering all aspects of protein microsequencing