A 6-week study with four college students as volunteer subjects was conducted for the purpose of evaluating the nutritional requirements of individuals undergoing stresses imposed by simulated aerospace conditions. A 4-day cycle diet of fresh foods was served at room temperature each day; it was comprised of 110 g of protein, 315 g of carbohydrate, 80 g of fat and 2621 calories. All the clinical data were in the normal range and no significant differences were observed between control and the confinement in the Life Support Systems Evaluator. Metabolic balances show excellent adjustment to the diet; all subjects were in positive nitrogen balance. Physiological measurements of heart rate, blood pressure and oral temperature were in the normal ranges. Minimal body weight changes were observed indicating that the diet was adequate.