This report provides a detailed account of the third meeting of the WHO Strategic Advisory Group of Experts on In Vitro Diagnostics (SAGE IVD) held in 2020. It discusses the development of the third WHO Model List of Essential In Vitro Diagnostics (EDL), including the methods used, progress updates, and recommendations for future directions. The report aims to guide global health policies and strategies related to in vitro diagnostics by offering expert advice and evidence-based recommendations. It is intended for policymakers, health organizations, and professionals involved in public health and diagnostic services.