Immobilized Cells: Basics and Applications

Progress in Biotechnology

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This publication contains full papers of both oral and poster presentations of the symposium "Immobilized Cells: Basics and Applications" that was held in Noordwijkerhout, The Netherlands, 26-29 November 1995. This volume covers recent developments in the field of immobilization e.g.: new support materials, characterization of support materials, kinetic characterizations, dynamic modelling, bioreactor types, scale up and applications are also given. Applications in the field of medicine, fermentation technology, food technology and environmental technology are described. Guidelines for research with immobilized cells. Based on the scientific sessions a strategy of research and methods for characterization of immobilized cells, especially in view of applications are given.

The goal was to relate basic research to applications and to extract guidelines for characterization of immobilized cells in view of process design and application from the contributions. The manuscripts presented in these proceedings give an extensive and recent overview of the research and applications of immobilized-cell technology.

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Additional Information

Publisher
Elsevier
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Published on
Mar 21, 1996
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Pages
842
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ISBN
9780080534473
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Language
English
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Genres
Science / Biotechnology
Science / Life Sciences / Cell Biology
Science / Life Sciences / Microbiology
Technology & Engineering / Biomedical
Technology & Engineering / Food Science / General
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Content Protection
This content is DRM protected.
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Pectins are one of the classes of complex structural plant cell wall polysaccharides. They are localized in the middle lamella and primary cell wall of higher plants. Pectins have a long-standing use as gelling agents whereas their enzymatic degradation or modification plays an important role in the processing of agricultural crops and the manufacturing of foods and beverages. Progress in pectin and pectinase research has been most prominent in two areas over the past 5 years. The first one concerns the analysis and elucidation of the complex chemical structure of pectin and identification of novel enzymes involved in the degradation of these structures. The second area concerns the mode of action and the 3-dimensional structure of various pectin degrading enzymes as well as the cloning of a large number of genes encoding enzymes involved in pectin degradation and modification. This book covers the following topics. First the structural, physical and chemical properties of pectin are treated followed by information about its biosynthesis and about the biological effects of pectin and its degradation products in biological systems such as plant-pathogen interactions and human nutrition. Identification of novel enzymes, the mode of action of different pectinases and the 3-D structure of bacterial pectate lyases forms the second block. This is followed by the genetics and regulation of pectinase biosynthesis in saprophytic and phytopathogenic microbial systems as well as in plant systems. Finally, developments in pectin manufacturing and application of pectinases in traditional (food, beverage) and novel technologies are treated. This book is meant for those actively involved in fundamental and applied aspects of pectin and pectinase research but it is also of value for those interested in plant cell wall biosynthesis and architecture, phytopathology, food technology and human nutrition. This book not only reflects the present status of research in the field but it will turn out to be a very useful reference work as well.
These proceedings contain most of the oral presentations and posters of the international symposium on Stability and Stabilization of Enzymes held in Maastricht in November 1992. They provide a comprehensive overview of the state-of-the-art in this field.


The possible applications of enzymes are enormous. Years of development have seen many enzymes brought onto the market, but they are still expensive to use. Therefore, their efficient application is a prerequisite for common usage. One of the main factors for this efficiency is the stability of the enzymes. The topics thus ranged from the extensive fundamental thermodynamic knowledge gathered in academic research to the practical applied knowledge built up in industry during the time that enzymes have been produced commercially.


The subject Stability and Stabilization of Enzymes was discussed from various points of view, as was reflected in the themes of the symposium sessions. In the session on Fundamentals of Enzyme Stabilisation the thermodynamic background of the phenomenon was highlighted. In yet another session, the recently developed analytical tools to measure enzyme stability and stabilisation were discussed. Further sessions comprised the physical, chemical and biological ways to obtain enzyme stabilisation and finally, the industrial practice of enzyme stabilisation was treated by representatives of the world's most important enzyme producers. The book will be of interest to researchers in universities and industry in the fields of biochemistry, enzymology and biotechnology.

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