This Standard specifies the requirements, test method, marking, labelling, instructions for use, packaging, transportation, storage, etc. of detection kit for antibodies to HIV-1 and HIV-2 (western blotting). This Standard applies to reagents for qualitative detection of antibodies to HIV-1 and HIV-2 using the western blotting method (including the recombinant immunoblotting method), to fix the multicomponent antigens or recombinant expression multicomponent antigens to the different locations of nitrocellulose membrane using the SDS-PAGA method or the direct sample application method, i.e. detection kits for antibodies to HIV-1 and HIV-2 (western blotting).